BSA 低脂肪酸，无蛋白酶，低内毒素

货号：BV1080-100g

规格：100克

价格：800元

贮存：4度贮存

运输：常温运输

有效期：3年

牛血清白蛋白（Bovine Serum Albumin, BSA）是牛血清中的一种球蛋白，又称第五组分（需要注意的是，第五组分并不是BSA的一种组份，而是Edwin J. Cohn教授早期从血液中通过特定的分离技术得到5种组份，其中第五种就是白蛋白），CAS号为9048-46-8，包含583个氨基酸残基，分子量为 66.430 kDa，等电点为4.7。牛血清白蛋白在生化实验中有广泛的应用，例如在western blot中作为封闭试剂。血液中的白蛋白主要起维持渗透压作用、PH缓冲作用、载体作用和营养作用。在动物细胞无血清培养中，添加白蛋白可起到生理和机械保护作用和载体作用。

低脂肪酸牛血清白蛋白产品及应用

牛血清白蛋白(低脂肪酸)，或者称为无脂肪酸牛血清白蛋白，或者称为Fat Acid Free BSA，产品不含有或含有极微量的脂肪酸。常规方法纯化的BSA难以有效去除其结合的脂肪酸。除了特别纯化的BSA产品外，市售的普通BSA试剂含脂肪酸量的范围一般是: 每mol BSA含0.2~1.5 mol脂肪酸。然而，一些生物医学研究要求使用不含游离脂肪酸的BSA，否则BSA所含高浓度的脂肪酸将影响细胞培养、分化、代谢反应、生化测定等。例如，在3T3-L1脂肪细胞分化过程中不能使用普通BSA试剂，否则影响分化和细胞代谢状态；在测定培养的组织细胞释放的游离脂肪酸浓度时，也必须用不含脂肪酸的BSA，否则难以准确测定甘油三酯水解和游离脂肪酸释放。

     BSA低脂肪酸产品是使用专利的热处理方法纯化而来的超纯粉末。经过专门的去脂纯化处理，脂肪酸含量低于0.05% (w/w)，性价比高，方便溶解。该无脂肪酸BSA可用于脂肪代谢相关分析的载体蛋白，也可与某种特异性的脂肪酸结合以形成白蛋白，可应用于增强细胞培养 体系的性能；若实验中脂肪或脂肪酸的存在影响激素或者胆固醇的读数时，这个产品是首选；还可以作为某些脂肪以及脂肪酸分析的替代品。

Bovine Serum Albumin

low FFA content, protease free,low endotoxin

General Description: Bovine Serum Albumin (BSA) is the major protein species found in bovine blood plasma and has applications in life science disciplines such as cell culture, in-vitro diagnostics, human and veterinary pharmaceuticals, molecular biology, serology and general research.

Bovogen Biologicals' BSA (Fraction V) is manufactured using a proprietary heat shock process under GMP conditions. The  BSA purity is consistently measured at 99 – 100%.

The BSA is a premium grade product that is ideal for a variety of applications requiring a product with high protein purity and low endotoxin. It is also fatty acid free, IgG free, protease free and is bovine virus free.
Raw Material Origin: Manufactured from the plasma of animals of 100% Australian origin that have undergone veterinary ante- and post-mortem inspection and have been approved for human consumption. 
Appearance: Pale-cream to white coloured, lyophilized powder.
Applications: 

• general additive in growth media for tissue and cell culture
• protein standard
• general research or diagnostic assay reagent
• assays requiring  low endotoxin levels 
• additive in specific diluents
• vaccine production
• blocking agent in immunoassays
• stabiliser in certain types of protein ligands
• reagent for protease sensitive immunoassays
• hybridization
• additive in serum reduced or serum free media
• additive in fatty acid sensitive cell culture systems
• additive to assist in immunohaemaglutination

Storage and Handling: Product is shipped at ambient temperature however, recommended storage is at 2-8°C.

引用BSA发表文章列表（6篇）：

1. [2014 IF=1.24] Water-soluble protein molecular weight istribution and effects on wheat malt quality uring malting.

Journal: J. Inst. Brew.  2014; 120: 399–403

Authors: Lan Xie, Yuhong Jin, Jinhua Du and Kaili Zhang

Institution：College of Food Science and Engineering, Shandong Agricultural University

Paper link： https://doi.org/10.1002/jib.182

2. [2021 IF=6.95] Protein FT-IR amide bands are beneficial to bacterial typing.

Journal: International Journal of Biological Macromolecules 2022; 207: 358–364

Authors: Jinghang Xiea, Fan Yang, Haimei Shia, Jintao Yana, Hao Shena, Shaoning Yua, Ning Gana, Bin Fenga, Li Wang.

Institution：School of Material Science and Chemical Engineering, Ningbo University

Paper link：https://linkinghub.elsevier.com/retrieve/pii/S0141813022004172

3. [2021 IF=7.96] Developing a novel Bifidobacterium phage quantitative polymerase chain reaction-based assay for tracking untreated waste.

Journal: Science of The Total Environment Volume 838, Part 2, 10 September 2022, 155815

Authors: XiangLi, Warish Ahmed，Ziqi Wu，Yu Xia

Institution：Southern University of Science and Technology

Paper link：https://linkinghub.elsevier.com/retrieve/pii/S0048969722029126

4. [2022 IF=3.9] The effect of barley to wheat ratio inmalt blends on protein composition and physicochemical characteristics of wort and beer.

Journal: J. Food Sci. 2023;88:193–203.

Authors: Xiu Li，Junhua Yang，Lan Xie，Yuhong Jin，Junhan Liu，Wenjiao Xing

Institution：Shandong Agricultural University

Paper link：https://ift.onlinelibrary.wiley.com/doi/abs/10.1111/1750-3841.16428

5. [2022 IF=5.3] Immunofuorescence staining of chloroplast proteins with frozen sections of plant tissues.

Product: BSA Cat:BA0332

Journal: Plant Cell Reports (2024) 43:168

Authors: Lulu Wang,Fang Zeng,Yongjuan Jiao, Qingqing Zhou,Jinjie An,Hongbo Gao

Institution：College of Biological Sciences and Technology, Beijing Forestry University

Paper link：https://doi.org/10.1007/s00299-024-03255-2

6. [2023 IF=6] DRD2-Mediated AMPK Ubiquitination Regulates the Occurrence of Hepatic Steatosis.

Product: BSA Cat:BV1080

Journal: Liver International, 2025 45:e70053

Authors: Peng Ma, Hao Ou. Junze Cai, Yuanli Zhang, Yu Ou

Institution：School of Life Science and Technology, China Pharmaceutical University

Paper link：https://doi.org/10.1111/liv.70053