|
|
酶
|
寡核苷酸序列
|
切割率 %
|
|
|
2 hr
|
20 hr
|
|
|
Acc I
|
G GTCGAC C
CG GTCGAC CG
CCG GTCGAC CGG
|
0
0
0
|
0
0
0
|
|
|
Afl III
|
C ACATGT G
CC ACATGT GG
CCC ACATGT GGG
|
0
>90
>90
|
0
>90
>90
|
|
|
Asc I
|
GGCGCGCC
A GGCGCGCC T
TT GGCGCGCC AA
|
>90
>90
>90
|
>90
>90
>90
|
|
|
Ava I
|
C CCCGGG G
CC CCCGGG GG
TCC CCCGGG GGA
|
50
>90
>90
|
>90
>90
>90
|
|
|
BamH I
|
C GGATCC G
CG GGATCC CG
CGC GGATCC GCG
|
10
>90
>90
|
25
>90
>90
|
|
|
Bgl II
|
C AGATCT G
GA AGATCT TC
GGA AGATCT TCC
|
0
75
25
|
0
>90
>90
|
|
|
BssH II
|
G GCGCGC C
AG GCGCGC CT
TTG GCGCGC CAA
|
0
0
50
|
0
0
>90
|
|
|
BstE II
|
G GGT(A/T)ACC C
|
0
|
10
|
|
|
BstX I
|
AACTGCAGAA CCAATGCATTGG
AAAACTGCAG CCAATGCATTGG AA
CTGCAGAA CCAATGCATTGG ATGCAT
|
0
25
25
|
0
50
>90
|
|
|
Cla I
|
C ATCGAT G
G ATCGAT C
CC ATCGAT GG
CCC ATCGAT GGG
|
0
0
>90
50
|
0
0
>90
50
|
|
|
EcoR I
|
G GAATTC C
CG GAATTC CG
CCG GAATTC CGG
|
>90
>90
>90
|
>90
>90
>90
|
|
|
Hae III
|
GG GGCC CC
AGC GGCC GCT
TTGC GGCC GCAA
|
>90
>90
>90
|
>90
>90
>90
|
|
|
Hind III
|
C AAGCTT G
CC AAGCTT GG
CCC AAGCTT GGG
|
0
0
10
|
0
0
75
|
|
|
Kpn I
|
G GGTACC C
GG GGTACC CC
CGG GGTACC CCG
|
0
>90
>90
|
0
>90
>90
|
|
|
Mlu I
|
G ACGCGT C
CG ACGCGT CG
|
0
25
|
0
50
|
|
|
Nco I
|
C CCATGG G
CATG CCATGG CATG
|
0
50
|
0
75
|
|
|
Nde I
|
C CATATG G
CC CATATG GG
CGC CATATG GCG
GGGTTT CATATG AAACCC
GGAATTC CATATG GAATTCC
GGGAATTC CATATG GAATTCCC
|
0
0
0
0
75
75
|
0
0
0
0
>90
>90
|
|
|
Nhe I
|
G GCTAGC C
CG GCTAGC CG
CTA GCTAGC TAG
|
0
10
10
|
0
25
50
|
|
Not I
|
TT GCGGCCGC AA
ATTT GCGGCCGC TTTA
AAATAT GCGGCCGC TATAAA
ATAAGAAT GCGGCCGC TAAACTAT
AAGGAAAAAA GCGGCCGC AAAAGGAAAA
|
0
10
10
25
25
|
0
10
10
90
>90
|
|
Nsi I
|
TGC ATGCAT GCA
CCA ATGCAT TGGTTCTGCAGTT
|
10
>90
|
>90
>90
|
|
Pac I
|
TTAATTAA
G TTAATTAA C
CC TTAATTAA GG
|
0
0
0
|
0
25
>90
|
|
Pme I
|
GTTTAAAC
G GTTTAAAC C
GG GTTTAAAC CC
AGCTTT GTTTAAAC GGCGCGCCGG
|
0
0
0
75
|
0
25
50
>90
|
|
Pst I
|
G CTGCAG C
TGCA CTGCAG TGCA
AA CTGCAG AACCAATGCATTGG
AAAA CTGCAG CCAATGCATTGGAA
CTGCAG AACCAATGCATTGGATGCAT
|
0
10
>90
>90
0
|
0
10
>90
>90
0
|
|
Pvu I
|
C CGATCG G
AT CGATCG AT
TCG CGATCG CGA
|
0
10
0
|
0
25
10
|
|
Sac I
|
C GAGCTC G
|
10
|
10
|
|
Sac II
|
G CCGCGG C
TCC CCGCGG GGA
|
0
50
|
0
>90
|
|
Sal I
|
GTCGAC GTCAAAAGGCCATAGCGGCCGC
GC GTCGAC GTCTTGGCCATAGCGGCCGCGG
ACGC GTCGAC GTCGGCCATAGCGGCCGCGGAA
|
0
10
10
|
0
50
75
|
|
Sca I
|
G AGTACT C
AAA AGTACT TTT
|
10
75
|
25
75
|
|
Sma I
|
CCCGGG
C CCCGGG G
CC CCCGGG GG
TCC CCCGGG GGA
|
0
0
10
>90
|
10
10
50
>90
|
|
Spe I
|
G ACTAGT C
GG ACTAGT CC
CGG ACTAGT CCG
CTAG ACTAGT CTAG
|
10
10
0
0
|
>90
>90
50
50
|
|
Sph I
|
G GCATGC C
CAT GCATGC ATG
ACAT GCATGC ATGT
|
0
0
10
|
0
25
50
|
|
Stu I
|
A AGGCCT T
GA AGGCCT TC
AAA AGGCCT TTT
|
>90
>90
>90
|
>90
>90
>90
|
|
Xba I
|
C TCTAGA G
GC TCTAGA GC
TGC TCTAGA GCA
CTAG TCTAGA CTAG
|
0
>90
75
75
|
0
>90
>90
>90
|
|
Xho I
|
C CTCGAG G
CC CTCGAG GG
CCG CTCGAG CGG
|
0
10
10
|
0
25
75
|
|
Xma I
|
C CCCGGG G
CC CCCGGG GG
CCC CCCGGG GGG
TCCC CCCGGG GGGA
|
0
25
50
>90
|
0
75
>90
>90
|